Journal: Scientific Reports

Article Title: Cell passage number drives transcriptomic drift as an overlooked factor in experimental reproducibility

doi: 10.1038/s41598-025-29424-1

Figure Lengend Snippet: RNA-seq expression profiling of ACHN and Renca cell lines at different passage numbers. ( A , B ) Violin plots showing the distribution of gene expression levels in ACHN ( A ) and Renca ( B ) cells at different passages (P3, P10/P11, P17, P24, P39). Each group demonstrates consistent intra-sample expression distribution. ( C , D ) Principal component analysis (PCA) illustrating the overall trajectory of transcriptomic changes in ACHN ( C ) and Renca ( D ) cells across passages. Each point represents an individual sample, with colors indicating different passage time points. ( E , F ) Pearson correlation heatmaps depicting gene expression correlations among all samples for ACHN ( E ) and Renca ( F ) cells.

Article Snippet: ACHN and Renca cell lines were purchased from Procell Life Science & Technology Co., Ltd. (originating from ATCC) and authenticated by STR profiling.

Techniques: RNA Sequencing, Expressing, Gene Expression

Journal: Scientific Reports

Article Title: Cell passage number drives transcriptomic drift as an overlooked factor in experimental reproducibility

doi: 10.1038/s41598-025-29424-1

Figure Lengend Snippet: Quantitative changes of DEGs in ACHN and Renca cells at different passages compared to P3. Number of DEGs in ACHN cells at P10, P17, P24, and P39 passages relative to P3. Number of DEGs in Renca cells at P11, P17, P24, and P39 passages relative to P3. Red bars represent up-regulated genes (UP), blue bars indicate down-regulated genes (DOWN), with numerical values showing the quantitative changes of DEGs.

Article Snippet: ACHN and Renca cell lines were purchased from Procell Life Science & Technology Co., Ltd. (originating from ATCC) and authenticated by STR profiling.

Techniques:

Journal: Scientific Reports

Article Title: Cell passage number drives transcriptomic drift as an overlooked factor in experimental reproducibility

doi: 10.1038/s41598-025-29424-1

Figure Lengend Snippet: Cluster analysis of DEGs across passages. Heatmap of representative DEGs in ACHN cells (n = 2) at P3, P10, P17, P24, and P39 passages. Heatmap of representative DEGs in Renca cells (n = 2) at P3, P11, P17, P24, and P39 passages. Shown is a heatmap of Z-scores (based on log 10 (FPKM + 1) values) for representative DEGs. Genes were clustered by Euclidean distance. Red and blue colors indicate up- and down-regulation, respectively.

Article Snippet: ACHN and Renca cell lines were purchased from Procell Life Science & Technology Co., Ltd. (originating from ATCC) and authenticated by STR profiling.

Techniques: